Tertiary conformational transition in sheep hemoglobins induced by reaction with 5,5´-dithiobis(2-nitrobenzoate) and by binding of inositol hexakisphosphate

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We have determined the second-order reverse rate constant, kR, for the reaction of 5,5´-dithiobis(2-nitrobenzoate) – DTNB – with sheep hemoglobins as a function of pH from values of the second-order forward rate constant, kF, and the equilibrium constant, Kequ, at 25 °C: kR¼ kF Kequ. We demonstrate that (i) inositol hexakisphosphate (inositol-P6) decreases kF and kR by increasing Krt,...

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KO Okonjo, I Adeogun & JO Babalola

Okonjo Kehinde » Born 1942. Educated King's College, Lagos (1957-63). BSc, PhD (University of Ibadan). Retired Professor of Chemistry, University of Ibadan. Currently Professor of Chemistry, Covenant University. Current research interests: Kinetics and thermodynamics of reactions of reaction of Ellman's reagent with hemoglobin sulphydryl groups. Publications in such high impact journals as the Journal of... view full profile

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