Kinetic study and characterization of 1,4--endoglucanase of Aspergillus niger ANL301

Kinetic study and characterization of 1,4--endoglucanase of Aspergillus niger ANL301

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Submerged fermentation of Aspergillus niger ANL 301, in basal medium containing cellulose as sole carbon source, yielded crude extracellular proteins with 0.54 ± 0.02 units mg protein-1 of 1, 4- β-endoglucanase activity. Partial purification by ammonium sulphate precipitation (80% saturation) and gel filtration on Sephadex 25-300 gave two active fractions of 1, 4- β-endoglucanase, which...
 
Published at Dynamic Biochemistry, Process Biotechnology and Molecular Biology, Global Science Books, Japan.
Volume 5(2)
Pages 1-6
 
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Shalom Nwodo Chinedu, O.C. Nwinyi, U.A. Okafor and V.I. Okochi
Chinedu Shalom » Shalom Nwodo Chinedu, a Professor of Biochemistry and Molecular Biology, is a faculty in the Department of Biochemistry, College of Science and Technology, Covenant University, Ota, Nigeria. Professor Chinedu is a seasoned academic, researcher and University administrator. He has been actively engaged in University education since 1992. He is the immediate past Director of the African... view full profile
Chinedu Shalom
 
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